Law of Segregation is the second Law of Inheritance given by Mendel which was
formulated based on the observations noted down while performing Monohybrid
cross.
When self-hybridization of F1 progeny is performed, we could see that in F2
generation, the phenotypic ratio is obtained as 3 : 1 (Tall : Dwarf) whereas
genotypic ratio is obtained as 1 : 2 : 1 (Pure tall : Hybrid tall : Pure
recessive).
Thus, we could observe that the recessive character which disappeared
in F1 generation, reappears in F2 generation and shows that alleles or genes do not mix with one another and separated pure
during gamete formation
which is Law of Segregation or Law of Purity of Gametes.
